Doug Crawford
Director of Marine Genomics
Rosenstiel School of Marine and Atmospheric Science
University of Miami
4600 Rickenbacker Causeway
Miami, FL 33149-1098 USA
305-361-4121
Web Site:
http://www.rsmas.miami.edu/divs/mbf/people/dcrawford.htm
http://funnybase.umkc.edu
Biography
Education
| B.A. | (1980) | Zoology, University of Washington, Seattle WA |
| M.Sc. | (1983) | Physiological Ecology, University of Kansas, Lawrence KS. |
| Ph. D. | (1989) | Biology, Johns Hopkins University, Baltimore MD |
| Post-Doc | (1991) | Hopkins Marine Station, Stanford University, Pacific Grove, CA. |
Research Interests
My laboratory is using a genomic approach to study how hearts work and why some hearts work better than others. My research combines molecular, physiological and evolutionary studies to determine the adaptive importance of gene expression and how changes in genes affect cardiac performance. Our studies of gene expression and heart function use a small minnow (Fundulus, mummichog, killifish). We use this fish because it has population structure similar to humans (large structured populations) and there are functional differences in heart function. For example, fish from the colder waters in Maine utilize sugar (glucose) more rapidly than fish in the warmer water off Georgia. We can use genomic tools to investigate many thousand of genes at once to determine how this and other differences in cardiac function are accomplished.Recent Publication
Crawford, D. L., V. A. Pierce and J. A. Segal. Evolutionary physiology of closely related taxa: analyses of enzyme expression. Am. Zool 1999. 32 : 389-400
Crawford, D. L., J. A. Segal, and J. L. Barnett.. Evolutionary analysis of TATA-less proximal promoters. Molecular Biology and Evolution1999 16:194-207.
Segal, J. A., J. L. Barnett and D. L. Crawford. Functional analyses of natural variation in Sp1 binding sites of a TATA-less promoter. J. Mol. Evol. 1999 49:736-749
Podrabsky, J. E., C. Javillonar, S. C. Hand and D. L. Crawford 2000. Intraspecific Variation in Aerobic Metabolism and Glycolytic Enzyme Expression in Heart Ventricles from Fundulus heteroclitus. Am J. Physiology 2000 279:R2344-R2348.
Crawford, D.L. Functional genomics does not have to be limited to a few select organisms. GenomeBiology 2001 http://www.genomebiology.com/2001/2/1/interactions/1001/(2001).
Oleksiak, M.F., Kolell, K. & Crawford, D.L. The utility of natural populations for microarray analyses: isolation of genes necessary for functional genomic studies. Marine Biotechnology 2001 3:S203-S211
Kolell, K.J. & Crawford, D.L. Evolution of Sp Transcription Factors. Molecular & Biology and Evolution 2002 19: 116-222.
Oleksiak, M.F., Churchill, G. & Crawford, D.L. Variation in gene expression within and among natural populations. Nature Genetics, 2002. 32: p. 261-266.
Crawford, D.L. Evolution of physiological adaptation, in Cell and Molecular Responses to Stress, K.B. Storey and J.M. Storey, Editors. 2002, Elsevier Publishing: NY.
Oleksiak, M., F. and Crawford, D. L., 5' genomic structure of human Sp3. Molecular Biology & Evolution, 2002. 19: 2026-2029