ARCH Program Facility Core
Contact reink@fiu.edu
The Facility
Core serves as a support center for research projects currently on-going
under the auspices of the ARCH program at FIU.
It is the main repository and supplier of microorganism cultures,
purified toxins, DNA and RNA, la
beled toxins and antibodies for a variety of investigators.
In addition, it supplies access to a wide range of equipment,
and a number of services related to marine toxin
research.
The Facility Core has recently completed major renovations. The Core
has moved to a new laboratory (OE316). This space was remodeled to provide
200 sq. ft. of floor space dedicated to cultures. High density shelving
increased shelf space to 200 sq. ft., and we estimate our capacity will
be 2000L. The renovations included backup temperature control, programmable
lighting and air ports for each of ten shelves. These renovations were
funded in part by the Center
of Excellence in Marine Biotechnology grant from the State of Florida.
The FIU College of Arts and Sciences paid for planning and a portion
of the renovations. An adjacent 100 sq. ft. lab is used for media preparation,
and 600 sq. ft. of general laboratory space houses the core instrumentation
and is used for cell harvesting, DNA/ RNA and toxin isolation.
Microorganisms
currently in culture
(click
on code for more details):
| CODE |
GENUS |
SPECIES |
STRAIN or SOURCE* |
AVAILABILITY |
| Alexandrium |
monilatum |
EPA |
n/a |
|
| Amphidinium |
carterae |
CCMP 1314 |
yes |
|
| Arthronema |
(unidentified) |
21-10-20 |
n/a |
|
| Chattonella |
subsalsa |
CCMP 217 |
yes |
|
| Heterocapsa |
triquetra |
CCMP 448 |
yes |
|
| Heterosigma |
akashiwo |
CCMP 1870 |
yes |
|
| Karenia |
brevis |
CCMP 718 |
yes |
|
| Karenia |
brevis |
CCMP 2228 |
yes |
|
| Karenia |
brevis |
CCMP 2229 |
yes |
|
| Karenia |
brevis |
CCMP 2281 |
yes |
|
| Karenia |
brevis |
EPA JR |
yes |
|
| Karenia |
brevis |
EPA SH/RE |
n/a |
|
| Lyngbya |
(unidentified) |
15-2 |
yes |
|
| Microcystis |
aeruginosa |
UTCC 299 |
yes |
|
| Prymnesium |
parvum |
CCMP 1926 |
yes |
|
| Prymnesium |
parvum |
Yasumoto |
yes |
|
| Prorocentrum |
hoffmanianum |
CCMP 683 |
yes |
|
| Prorocentrum |
lima |
FL bay |
yes |
|
| Prorocentrum |
micans |
CCMP 1591 |
yes |
|
| Prymnesiophyceae |
(unidentified) |
CCMP 1212 |
yes |
|
| Pyrocystis |
lunula |
CSEO |
yes |
|
| Rhodomonas |
salina |
CCMP 1319 |
yes |
|
| Symbiodinium |
(unidentified) |
CALBIO 133 |
yes |
|
| Synechococcus |
(unidentified) |
CCMP 837 |
yes |
*Source
abbreviations:
CCMP = Provasoli-Guillard National
Center for Culture of Marine Phytoplankton
EPA = Environmental Protection Agency, Gulf Breeze, FL
UTCC = University of Toronto Culture
Collection
CSEO = Center for Science Education+Outreach,
University of Utah
CALBIO = CalBioMarine Technologies, Inc.
BioTek mQuant microplate spectrophotometer
FastPrep DNA machine
HP1050 HPLC
ThermoFinnigan HPLC with UV and fluorescence detectors
MJ Opticon Thermal Cycler
Zeiss Axiovert Microscope
Biotage Sp1 automated flash chromatography
system
LabScale Tangential Flow Filtration system
Services
available:
Toxin extraction & purification
ELISA for okadaic acid and brevetoxin
Protein phosphatase inhibition assays for toxins
DNA/RNA extraction
For
more information, contact:
Lori
Mason
305
348-6837
masonl@fiu.edu
Dr.
Kathleen Rein
305-348-6682
reink@fiu.edu
Copyright
2005. University of Miami & Florida International University,
All Rights Reserved.
The
ARCH Program is funded by the
National Institute of
Environmental Health Sciences
Grant Number: S11 ES11181
